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Home » Biology Homework Help » Biotechnology » Bi-and Poly-Functional Enzymes
Bi-and Poly-Functional Enzymes
These enzymes are produced by genes obtained by fusion of the coding regions of two or more genes encoding different enzymes. In simple, terms, the strategy for production of such enzymes is as follows. Suppose gene a produces enzyme A and gene b produces enzyme B, and for some reason it is desirable to bring the two enzyme activities together. The stop codon, e.g. TAG, of gene a is deleted, and this modified gene a is then joined with the TAG or initiation codon of gene b. It needed, a linker sequence encoding 2-10 amino acids may be introduced between the genes a and b. It is essential that the fusion of the two genes does not change the reading frame of gene b, i.e. the two genes are fused in the correct reading frame. The fused gene will produce a single polypeptide which will possess/exhibit the activities of both the enzymes A and B.

Several bi- and multifunctional enzymes have been, produced; some of the examples are: -galactosidase-galactokinase, -galactosidase-galactose dehydrogenase, -galactokinase-galactose dehydrogenase-galactokinase, galactose dehydrogenase-luciferase. Such enzymes provide the advantage that two or more enzyme activities required in sequence in a metabolic pathway are located in close proximity, i.e. in the same molecule, which may considerably enhance the efficiency of the pathway. These enzymes are useful in preparation of biosensors when two or more enzymes need to be immobilized together for enhanced efficiency in detection and in metabolic engineering, and are easy to separate and purify (during downstream processing).

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